The aim of this study was to develop and validate a high-performance liquid chromatographic-tandem mass spectrometric (LCMS/\nMS) method for analysis of the amiridine in human plasma. The analyte and internal standard (IS), zolpidem, were extracted\nfrom human plasma by solid phase extraction (SPE with SOLA cartridges) and separated on a Zorbax SB-C18 column using\nmethanol and 0.2% formic acid in water as mobile phase. Detection was performed using an electrospray ionization source and\nmass spectrometric positive multireaction monitoring mode (+MRM) at a voltage capillary of +2000V. The assay was linear over\nthe concentration range 0.5ââ?¬â??200 ng/mL with the lowest limit of quantification (LLOQ) of 0.5 ng/mL. The method also afforded\nsatisfactory results in terms of the sensitivity, specificity, precision (intra- and interday CV < 12%), accuracy, recovery, and the\nstability of the analyte under various conditions. The method can be successfully applied to pharmacokinetic studies.
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